SISU Hospital

Gamates (ovum and sperm) Rejuvination by Stemcell and PRP treatment

Ovum Freezing

Gamates are an organism’s reproductive cells. They are also referred to as sex cells.  Female gametes are called ova or egg cells, and male gametes are called sperm.  Gamates are haploid cells, and each cell carries only one copy of each chromosome.

 In Many conditions, both male and female loose their sexual cells rapidly. In women it is called pre mature ovaraian failure, in male it is called Azoospermia or testicular failure. Previously, if the patient had above said problem they have to undergo oocyte or sperm donation. Now a days, many therapeutic options are available to improve the oocyte count and sperm count. Due to scientific advancement, stemcell treatment and PRP treatments are available to treat above said conditions. No. of studies are giving promising results. Eventhough, it is not guaranteed,before going to donor gamates patient can try this treatment.

Quantitative and qualitative functional improvement of ovarian and testicular function after injection of autologous PRP treatment. Ovarian rejuvenation means stimulating the ovaries to produce news eggs. So, PRP treatment can offer a new hope in the treatment of rejuvenating gamete. 

 

PRP(plasma rich in platelets) is a natural product where a high level of platelets is concentrated, but with growth factors concentration 3 to 5 times greater than plasma. Growth factors are stored in granules, and include platelet derivate growth factor (PDGFs), Transforming growth factor-beta(TGF-Beta), vessel endothelial growth factor(VEGF), epidermal growth factor(EGF), fibroblast growth factor(FGF), e insulin growth factor (IGF) and some other. These growth factors stimulate the oocyte production and sperm production. 

The protocol for gamete Rejuvenation(ovary and sperm):

1. Preparation of PRP – This begins from drawing blood from the patients
and isolate the PRP by centrification .
2. The second stage is injection of PRP for women to both ovaries either by Laproscopic or transvaginal ultrasound guidance. For men, it is injuected in both testis.

Indication for PRP therapy:

In Women:

  • Pre mature ovarian failure
  • Advanced Maternal age.
  • Post chemo therapy and radiotherapy.
  • Endometrial hypoplasia
  • Recurrent abortion
  • Recurrent IVF failure
  • Ashermen’s syndrome

In all these case PRP treatment significantly improves the oocyte quality and quantity, thus embryo quality and also endometrial growth.

In Men:

  • Very low spermatozoa (<10 million/ml)
  • Azoospermia
  • SOTA(Severe oligoterato asthenozoospermia)
  • Failed Hormonal treatment

PRP treatment in men, activate spermatogenesis and improve the testostrone level in the testicles. So, PRP is injected into the seminiferous tubules and stroma.

Improvement can be assured by:

  1. Increase in the AMH level.
  2. Decreasing FSH and LH and E2 level
  3. Getting menstruation
  4. In men, decreasing FSH and LH, improving testostera
  5. In thin endometrium, increasing the endometrial growth more than 7mm.

This procedure is innovative and due to its recent appearance, still experimental. However, according to currently available data, it succeeds in about 50 to 60 percent. In our hospital, we are done more than 100 patients, fertility restore in 40 to 45 percent of the patients.