Intracytoplasmic Sperm Injection(ICSI) is a specialized form of in Vitro Fertilization(IVF) that is used primarily for the treatment of severe form of SOTA and Azoospermia. In that after oocyte retrieval sperms are retrieved from the male reproductive tract using special techniques (MESA, PESA, TESE,TESA) Then, Morphologically good sperm selected and injected into the Oocyte and fertilized.